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Figure 3: Effect of ATF7 on the migration of MCF-10A, SKBR3, and MDA-MB-453 cells. MCF-10A cells were transfected with HER2-recombinant plasmid alone or with the combination of HER2- and ATF7-recombinant plasmids. Cell migration was determined by Transwell chamber assays. Relative quantification of migrated cells is shown in the right panel. (a) The mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (b) SKBR3 cells were transfected with ATF7-recombinant plasmid or ATF7 shRNA. Cell migration was determined by Transwell assays. Relative quantification of migrating cells is shown in the right panel, (c) the mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (d) scale bar, 50 μm. MDA-MB-453 cells were transfected with ATF7-recombinant plasmid or ATF7 shRNA. Cell migration was determined by Transwell assays. Relative quantification of migrated cells is shown in the right panel, (e) the mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (f) scale bar, 25 μm. *,#P < 0.05, **,##P < 0.01. ATF7 = Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, MMP9 = Matrix metalloproteinase 9, uPA = Uridylyl phosphate adenosine, PAI-1 = Plasminogen activator inhibitor-1, qRT-PCR = Quantitative real-time polymerase chain reaction

Figure 3: Effect of <i>ATF7</i> on the migration of MCF-10A, SKBR3, and MDA-MB-453 cells. MCF-10A cells were transfected with HER2-recombinant plasmid alone or with the combination of HER2- and <i>ATF7</i>-recombinant plasmids. Cell migration was determined by Transwell chamber assays. Relative quantification of migrated cells is shown in the right panel. (a) The mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (b) SKBR3 cells were transfected with <i>ATF7</i>-recombinant plasmid or <i>ATF7</i> shRNA. Cell migration was determined by Transwell assays. Relative quantification of migrating cells is shown in the right panel, (c) the mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (d) scale bar, 50 μm. MDA-MB-453 cells were transfected with <i>ATF7</i>-recombinant plasmid or <i>ATF7</i> shRNA. Cell migration was determined by Transwell assays. Relative quantification of migrated cells is shown in the right panel, (e) the mRNA expression of MMP9, MMP2, uPA, and PAI-1 as measured by qRT-PCR, (f) scale bar, 25 μm. *<sup>,#</sup><i>P</i> < 0.05, **<sup>,##</sup><i>P</i> < 0.01.<i> ATF7 </i>= Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, MMP9 = Matrix metalloproteinase 9, uPA = Uridylyl phosphate adenosine, PAI-1 = Plasminogen activator inhibitor-1, qRT-PCR = Quantitative real-time polymerase chain reaction