Figure 1: ATF7 expression in breast cancer. (a) ATF7 expression in different molecular subtypes of breast cancer. *Normal basal-like versus luminal A, HER2-E or basal-like; ^#Luminal A versus HER2-E, (b) differential expression of ATF7 in HER2-negative and positive breast cancer samples as detected by IHC staining, (c) tissue microarray immunohistochemical staining of ATF7 in HER2-negative and positive breast cancer samples. Brown, ATF7-positive cells. Scale bar, 50 μm, (d) ATF7 and HER2 protein expression in MCF-10A normal mammary epithelial cells, MCF-7, and T47D cells, HER2-enriched SKBR3, and MDA-MB-453 cells as measured by WB analysis. Relative quantitative analysis of ATF7 protein expression is shown on the right, (e) transcriptional expression of ATF7 in MCF-10A, MCF-7, T47D, SKBR3, and MDA-MB-453 cells as measured by qRT-PCR. *HER2-enriched cell lines versus MCF-10A; ^#Luminal A subtype cells versus MCF-10A; ^@@ HER2-enriched cell lines versus luminal A subtype cells. **^,##,@@P < 0.01. IHC = Immunohistochemistry, ATF7 = Activating transcription factor 7, HER2 = Human epidermal growth factor receptor 2, WB = Western blot, qRT-PCR = Quantitative real-time polymerase chain reaction