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Figure 2: Selenocystine induces S-phase arrest and apoptosis in JEG-3 cells. (a) Cell apoptosis and cell cycle distribution. Cells were seeded in 6-well plate and treated with 10, 20, and 40 μM selenocystine for 72 h. Then cells were collected, stained with propidium iodide solution, and examined by flow cytometry. (b) Statistic analysis of cell cycle distribution. Dose-dependent (c) and time-dependent (d) effects of selenocystine on cyclin A expression. The protein expression was detected by western blotting. (e) Statistic analysis of cell apoptosis. (f) Effects of selenocystine on cell apoptosis markers

Figure 2: Selenocystine induces S-phase arrest and apoptosis in JEG-3 cells. (a) Cell apoptosis and cell cycle distribution. Cells were seeded in 6-well plate and treated with 10, 20, and 40 μM selenocystine for 72 h. Then cells were collected, stained with propidium iodide solution, and examined by flow cytometry. (b) Statistic analysis of cell cycle distribution. Dose-dependent (c) and time-dependent (d) effects of selenocystine on cyclin A expression. The protein expression was detected by western blotting. (e) Statistic analysis of cell apoptosis. (f) Effects of selenocystine on cell apoptosis markers