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Figure 1: Selenocystine inhibits JEG-3 cell growth in vitro. (a) Dose-dependent inhibition of selenocystine against JEG-3 cell growth. Cells (6 × 103 cells/well) were seeded in 96-well plate and treated with 0–40 μM selenocystine for 72 h. (b) Time-dependent inhibition of selenocystine against JEG-3 cell growth. Cells were treated with 20 μM selenocystine for 12, 24, 48, and 72 h. Cell viability was detected with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. (c) Morphological changes. Cells were treated with 20 μM selenocystine for 72 h, and the cell morphology was observed under an inverted phase microscope (×200)

Figure 1: Selenocystine inhibits JEG-3 cell growth <i>in vitro</i>. (a) Dose-dependent inhibition of selenocystine against JEG-3 cell growth. Cells (6 × 10<sup>3</sup> cells/well) were seeded in 96-well plate and treated with 0–40 μM selenocystine for 72 h. (b) Time-dependent inhibition of selenocystine against JEG-3 cell growth. Cells were treated with 20 μM selenocystine for 12, 24, 48, and 72 h. Cell viability was detected with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. (c) Morphological changes. Cells were treated with 20 μM selenocystine for 72 h, and the cell morphology was observed under an inverted phase microscope (×200)