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Figure 2: Elevation of RhoE expression inhibiting cell growth and induced cell apoptosis in Ec-9706 cells. (a) Quantitative polymerase chain reaction analysis for RhoE mRNA expression level in RhoE-overexpressing Ec-9706 cells. β-actin was used as an internal control, *P < 0.05. (b) Ec-9706 cells transfected with expressing vector of nontagged RhoE were lysed and analyzed by immunoblotting using RhoE antibody. (c) Ec-9706 cells were transfected with the expression vector of RhoE or pcDNA3.1 control vector. Cell growth was evaluated every day by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide assay as described in the materials and methods section, **P < 0.01. (d) The effect of overexpressing RhoE on Ec-9706 cell apoptosis in vitro by cell flow cytometry, *P < 0.05

Figure 2: Elevation of RhoE expression inhibiting cell growth and induced cell apoptosis in Ec-9706 cells. (a) Quantitative polymerase chain reaction analysis for RhoE mRNA expression level in RhoE-overexpressing Ec-9706 cells. β-actin was used as an internal control, *<i>P</i> < 0.05. (b) Ec-9706 cells transfected with expressing vector of nontagged RhoE were lysed and analyzed by immunoblotting using RhoE antibody. (c) Ec-9706 cells were transfected with the expression vector of RhoE or pcDNA3.1 control vector. Cell growth was evaluated every day by 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazoliumbromide assay as described in the materials and methods section, **<i>P</i> < 0.01. (d) The effect of overexpressing RhoE on Ec-9706 cell apoptosis <i>in vitro</i> by cell flow cytometry, *<i>P</i> < 0.05