Journal of Cancer Research and Therapeutics Close
 

Figure 2: miR-451 inhibits cell proliferation in head and neck squamous cell carcinoma cell lines in vitro. Total levels of miR-451 in (a) KB cells transfected with the synthetic mimics of miR-451 or scrambled oligos, (d) HEp-2 cells transfected with an inhibitor of miR-451 or scrambled oligos. Real-time polymerase chain reaction was carried out to detect the expression of miR-451. U6B was used as an internal control. Cell counting assay over a 4-day period (b and e) and crystal violate staining method (c and f) were performed to access cell growth rates. Data are expressed as the mean ± standard error of the mean of the experiments performed in triplicate. *P < 0.05, **P < 0.01. The results showed that the forced expression of miR-451 suppressed cell proliferation, while the inhibition of miR-451 had the opposite effect

Figure 2: miR-451 inhibits cell proliferation in head and neck squamous cell carcinoma cell lines<i> in vitro</i>. Total levels of miR-451 in (a) KB cells transfected with the synthetic mimics of miR-451 or scrambled oligos, (d) HEp-2 cells transfected with an inhibitor of miR-451 or scrambled oligos. Real-time polymerase chain reaction was carried out to detect the expression of miR-451. U6B was used as an internal control. Cell counting assay over a 4-day period (b and e) and crystal violate staining method (c and f) were performed to access cell growth rates. Data are expressed as the mean ± standard error of the mean of the experiments performed in triplicate. <sup>*</sup><i>P</i> < 0.05, <sup>**</sup><i>P</i> < 0.01. The results showed that the forced expression of miR-451 suppressed cell proliferation, while the inhibition of miR-451 had the opposite effect