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Figure 1: miR-451 is downregulated in head and neck squamous cell carcinoma tissues and cell lines. (a) Real-time polymerase chain reaction analysis was performed to detect the expression of miR-451 in 50 cases of head and neck squamous cell carcinoma. Normal: Noncancerous matched tissues; tumor: Head and neck squamous cell carcinoma. miR-451 expression levels were calculated by the 2−△Ct method and normalized to U6 small nuclear RNA. The plot shows that the miR-451 expression reduced head and neck squamous cell carcinoma tissues. (b) Relative expression of miR-451 in eight head and neck squamous cell carcinoma cell lines, normal oral keratinocyte cell line, and dysplastic oral keratinocyte cell line

Figure 1: miR-451 is downregulated in head and neck squamous cell carcinoma tissues and cell lines. (a) Real-time polymerase chain reaction analysis was performed to detect the expression of miR-451 in 50 cases of head and neck squamous cell carcinoma. Normal: Noncancerous matched tissues; tumor: Head and neck squamous cell carcinoma. miR-451 expression levels were calculated by the 2<sup>−△Ct</sup> method and normalized to U6 small nuclear RNA. The plot shows that the miR-451 expression reduced head and neck squamous cell carcinoma tissues. (b) Relative expression of miR-451 in eight head and neck squamous cell carcinoma cell lines, normal oral keratinocyte cell line, and dysplastic oral keratinocyte cell line