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Figure 2: (a) Ascitic fluid cell block preparation showing atypical mesothelial cells with enlarged nuclei, prominent nucleoli, intracytoplasmic vacuolation, “cell-within-cell” arrangement along with “blebbing” of cell membranes (H and E, ×400). (b) Tumor cells showing diffuse and strong membranous positivity for mesothelin (HBME1) (DAB, ×400). (c) Staining for desmin highlighting an occasional reactive mesothelial cell. Tumor cells show complete negative staining (DAB, ×400). (d) Tumor cells showing distinct, focal nuclear positivity for p53 (DAB, ×400)

Figure 2: (a) Ascitic fluid cell block preparation showing atypical mesothelial cells with enlarged nuclei, prominent nucleoli, intracytoplasmic vacuolation, “cell-within-cell” arrangement along with “blebbing” of cell membranes (H and E, ×400). (b) Tumor cells showing diffuse and strong membranous positivity for mesothelin (HBME1) (DAB, ×400). (c) Staining for desmin highlighting an occasional reactive mesothelial cell. Tumor cells show complete negative staining (DAB, ×400). (d) Tumor cells showing distinct, focal nuclear positivity for p53 (DAB, ×400)