Year : 2022 | Volume
: 18 | Issue : 2 | Page : 352--361
Synergistic effect of growth factor receptor-bound protein 2/epidermal growth factor receptor dual-targeting peptide inhibitor and salinomycin on osteosarcoma
Zuochong Yu1, Yanlong Xu3, Longhai Du3, Fan Zhang2, Minghao Shao2, Lin Xie2, Guoping Cai4, Feizhou Lyu2
1 Department of Orthopedics, Huashan Hospital of Fudan University; Department of Orthopedics, Jinshan Hospital of Fudan University, Shanghai, China
2 Department of Orthopedics, Huashan Hospital of Fudan University, Shanghai, China
Context: The growth factor receptor-bound protein 2 (Grb2)-Sos1 interaction, mediated by modular domains, plays an essential role in the oncogenic MAPK signaling pathway in osteosarcoma (OS). Recently, a dual-targeting peptide that targets the epidermal growth factor receptor and Grb2-Src homology 3 domain in OS cells was designed and synthesized.
Aims: We investigated the synergistic effects of the peptide and salinomycin (Sal), a chemotherapeutic drug with effective anti-OS properties in clinical therapy.
Subjects and Methods: Flow cytometry was used to measure the targeting efficacy of the peptide. Migration and CCK-8 assays were used to explore whether Sal and the peptide could synergistically inhibit OS cell behavior. Western blotting was used to detect apoptosis.
Statistical Analysis Used: Data were analyzed using the GraphPad Prism 5.01. Statistical analysis was performed using the Student's t-test for the direct comparisons and one-way analysis of variance for the comparisons among the multiple groups. Statistical significance was set at P < 0.05.
Results: The peptide was shown to target OS cells. When applied together, Sal and the peptide synergistically inhibited OS cell migration, invasion, and proliferation through the inhibition of Grb2-Sos1. This synergistic treatment also promoted the apoptosis of OS cells and inhibited tumor volume in vivo.
Conclusions: These data provide valuable insights into the molecular mechanisms of OS and may be beneficial in clinical therapy.
No. 12, Middle Wulumuqi Road, Shanghai 200040
No. 1508, Longhang Road, Shanghai 201508
|How to cite this article:|
Yu Z, Xu Y, Du L, Zhang F, Shao M, Xie L, Cai G, Lyu F. Synergistic effect of growth factor receptor-bound protein 2/epidermal growth factor receptor dual-targeting peptide inhibitor and salinomycin on osteosarcoma.J Can Res Ther 2022;18:352-361
|How to cite this URL:|
Yu Z, Xu Y, Du L, Zhang F, Shao M, Xie L, Cai G, Lyu F. Synergistic effect of growth factor receptor-bound protein 2/epidermal growth factor receptor dual-targeting peptide inhibitor and salinomycin on osteosarcoma. J Can Res Ther [serial online] 2022 [cited 2022 Aug 20 ];18:352-361
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2022;volume=18;issue=2;spage=352;epage=361;aulast=Yu;type=0