Radiation-induced non-targeted effect <i>in vivo</i>: Evaluation of cyclooygenase-2 and endothelin-1 gene expression in rat heart tissues :Reza Fardid, Masoud Najafi, Ashkan Salajegheh, Elahe Kazemi, Abolhasan Rezaeyan, Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year : 2017 | Volume : 13 | Issue : 1 | Page : 51--55

Radiation-induced non-targeted effect in vivo: Evaluation of cyclooygenase-2 and endothelin-1 gene expression in rat heart tissues

Reza Fardid¹, Masoud Najafi², Ashkan Salajegheh¹, Elahe Kazemi¹, Abolhasan Rezaeyan³
¹ Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
² Department of Medical Physics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
³ Department of Medical Physics, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

Correspondence Address:
Masoud Najafi
Department of Medical Physics, School of Medicine, Tehran University of Medical Sciences, Tehran
Iran

Aim: In this study, we investigated expression levels of cyclooxygenase-2 (COX-2) and endothelin-1 (ET-1) genes after pelvis and heart irradiation in a rat model. These factors are involved in heart diseases (HDs). Materials and Methods: We used seven groups, including two groups of pelvic irradiation, two groups of whole body irradiation, two groups of heart irradiation, and one control nonirradiated group. Pelvis irradiations were conducted at a 2 cm × 2 cm in the pelvis area. Irradiation condition conducted using 1.25 MeV cobalt-60 gamma-rays (30 cGy/min). The changes at ET-1 and COX-2 gene expressions in heart tissue after pelvis and heart irradiation were measured and compared to the control and whole body irradiation groups at 24 h and 72 h after the exposure. Results: In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. Conclusion: This study reveals that nontargeted effect induced by radiation may be considered as an important phenomenon for induction of HD after radiotherapy.

How to cite this article:
Fardid R, Najafi M, Salajegheh A, Kazemi E, Rezaeyan A. Radiation-induced non-targeted effect in vivo: Evaluation of cyclooygenase-2 and endothelin-1 gene expression in rat heart tissues.J Can Res Ther 2017;13:51-55

How to cite this URL:
Fardid R, Najafi M, Salajegheh A, Kazemi E, Rezaeyan A. Radiation-induced non-targeted effect in vivo: Evaluation of cyclooygenase-2 and endothelin-1 gene expression in rat heart tissues. J Can Res Ther [serial online] 2017 [cited 2022 Aug 16 ];13:51-55
Available from: https://www.cancerjournal.net/article.asp?issn=0973-1482;year=2017;volume=13;issue=1;spage=51;epage=55;aulast=Fardid;type=0