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Evaluation of promoter hypermethylation of tumor suppressor gene BRCA1 in epithelial ovarian cancer


1 Department of Biochemistry, Maulana Azad Medical College, New Delhi, India
2 Department of Obstetrics and Gynaecology, Maulana Azad Medical College, New Delhi, India
3 Department of Biochemistry, All India Institute of Medical Sciences, Nagpur, Maharashtra, India
4 Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, India
5 Department of Pathology, Maulana Azad Medical College, New Delhi, India

Correspondence Address:
Dnyanesh B Amle,
Department of Biochemistry, All India Institute of Medical Sciences, Plot No. 2, Sector 20, MIHAN, Nagpur - 441 108, Maharashtra
India
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jcrt.JCRT_390_20

Context: Epithelial ovarian cancer (EOC) is a serious gynecological issue worldwide and its late detection is the major encumbrance in treatment procedures. Hypermethylation-mediated BRCA1 gene silencing results in failure of the repair system of damaged DNA playing an important role in ovarian carcinogenesis. BRCA1 gene hypermethylation can serve as a safe and highly specific clinical marker for EOC. Aims: The present study was conducted to evaluate the promoter hypermethylation of BRCA1 gene in EOC patients. Settings and Design: This hospital-based case–control study carried out in the tertiary care hospital in New Delhi. Subjects and Methods: Promoter hypermethylation of BRCA1 gene was examined in 30 EOC diagnosed untreated cases confirmed by histopathological examinations and compared with 30 normal healthy controls matched for age using methylation specific-polymerase chain reaction. Results: We found significantly higher BRCA1 promoter hypermethylation in the serum of EOC cases as compared to controls with P < 0.0001. BRCA1 gene methylation was found to have 70% sensitivity for the diagnosis of EOC with 100% specificity. A significant difference was observed in the range of CA125 levels, B12 and Folate levels between EOC cases and controls. Conclusions: We conclude that BRCA1 gene is significantly hypermethylated in EOC patients and thus can prove to be a noninvasive diagnostic tool. Our results provide prefatory evidence that epithelial ovarian epigenome can be influenced by dietary nutrients.


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    -  Das J
    -  Chandra L
    -  Gandhi G
    -  Amle DB
    -  Patnayak RL
    -  Khurana N
    -  Saxena A
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