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Evaluation of cytotoxic effects of fungal origin nanosilver particles on oral cancer cell lines: An in vitro study

1 Department of Conservative Dentistry & Endodontics, Al Badar Rural Dental College & Hospital, Kalaburgi, Karnataka, India
2 Department of Conservative dentistry & Endodontics, HKE's SN Institute of Dental Sciences and Research, Sedam Road, Kalaburgi, Karnataka, India
3 Consultant Endodontics, Director, RAK Dental Centre, Ras Al Khaima, UAE
4 Specialist Endodontics, Sharjah Specialty Dental Center, Sharjah, UAE
5 Department of Conservative Dentistry & Endodontics, SVS Institute of Dental Sciences, Mahbubnagar, Telangana, India
6 Department of Restorative, (Operative Dentistry), Jazan University, KSA, Saudi Arabia

Correspondence Address:
Kiran R Halkai,
Department of Conservative Dentistry and Endodontics, Al-Badar Rural Dental College and Hospital, Kalaburgi - 585 102
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jcrt.JCRT_1308_20

Background: Oral cancer is often associated with poor prognosis and it is found that conventional treatment options cause severe side effects, adjacent tissue disfigurement, and loss of function. Recently, silver nanoparticles (AgNPs) paved their path for cancer treatment. Aim: This study aimed to investigate cytotoxic effects of fungal procured AgNPs on oral squamous cell carcinoma (SCC-9) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Methodology: The silver nanoparticles were biosynthesized using the fungi Fusarium semitectum. Cell lines were cultured in a 1:1 ratio of Dulbecco's Modified Eagle's medium and Ham's F12 medium and subcultured in a T-75 cm2 flask. Cell count was adjusted to 1 × 105 cells/ml; 50,000 cells/well were seeded into a 96 well plate and incubated at 37°C, for 24 h in 5% CO2 humidified conditions. AgNPs (1.75–50 μl/ml) were added to the plates and further incubated at 37°C for another 24 h. Medium containing cells without AgNPs were used as a control group. Later, 20 μl of MTT was added to each well and incubated for 6 h at above-mentioned conditions. About 0.1 mL of Dulbecco's Modified Eagle's Medium solution was added to each well to solubilize formazan. The absorbance was measured using a Tecan reader at 540 nm. The experiment was repeated thrice independently. The percentage (%) inhibition of growth and the AgNP's concentration that prevents the cell growth by 50% (IC50) were determined. Results: Significant dose-dependent inhibition of the growth of SCC-9 cell lines was seen and IC50 was found at 12 μl/ml concentration of AgNPs. Conclusion: Biosynthesized AgNPs of fungal origin exhibit effective anticancer properties against the SCC-9 cell line.

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    -  Halkai KR
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    -  Patil S
    -  Alawadi J
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    -  Marukala NR
    -  Mohammad Albar NH
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