| ORIGINAL ARTICLE |
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| Year : 2015 | Volume
: 11
| Issue : 8 | Page : 239-243 |
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Effect of capilliposide for induction apoptosis in human nasopharyngeal cancer CNE-2 cells through up-regulating PUMA expression
Hua Yonghong, Hu Qiaoying, Piao Yongfeng, Tang Qiu, Feng Jiangguo
Department of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou 310022, China
Correspondence Address:
Hu Qiaoying
Department of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou 310022
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1482.170529
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Objective: To observe the apoptosis of capilliposide against human nasopharyngeal cancer CNE-2 cells and to study its primary mechanisms.
Materials and Methods: Vectors pSilencer-PUMA-small interfering RNA (siRNA) were constructed to transcribe functional siRNA specially targeting PUMA. The interfering plasmids were used to transfect CNE-2 cells with lipofectamine 2000 transfection reagent. PUMA messenger RNA (mRNA) expression levels were analyzed by polymerase chain reaction. The proliferation of CNE-2 cells was detected using MTT colorimetry. Annexin V/propidium iodide double staining was applied to detect the apoptosis rate of CNE-2 cells. The protein levels of p53, PUMA, and Bax were detected using Western blot analysis.
Results: Recombinant siRNA expression vector targeting PUMA was constructed. MTT assays showed capilliposide inhibited the proliferation of CNE-2 cells in a concentration-dependent manner. The inhibition was strengthened along with increased concentrations. Apoptosis detected by flow cytometry in control group, drug group, siRNA group, and drug combined siRNA group was 9.3 ± 2.3%, 31.4 ± 5.6%, 12.3 ± 4.1%, and 13.2 ± 3.7%, respectively. After pretreated by capilliposide, PUMA protein was upregulated, and BAX was distributed to mitochondria in CNE-2 cells using Western blot analysis, but this effect can be interrupted by PUMA-siRNA.
Conclusions: Capilliposide could induce the apoptosis of CNE-2 cells, which might be related with the increasing in PUMA-Bax pathway. |
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